The MLT (ASCP) certification exam is the most widely recognized credentialing exam for medical laboratory technicians. Passing the MLT exam is the final step to becoming a certified medical laboratory technician and getting licensed by the state where you’ll start your new career.

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1. Each of the drugs/drug classes listed above have been known to cause drug-induced hemolytic anemia, although cephalosporins are the MOST COMMON cause. Cephalosporins can cause drug-induced hemolytic anemia when a patient produces antibodies to the particular cephalosporin drug in the presence of red blood cells. The drugs can alter the membrane appearance of the red blood cells, causing the body to mistake them as foreign. Complement becomes activated due to these antibodies; red cells are then destroyed causing hemolytic anemia. Dark urine, caused by intravascular hemolysis, is one of the most common symptoms associated with this condition.

Which one of the following drugs/drug classes is the MOST COMMON cause of drug-induced hemolytic anemia?

2. The molecular weight of a hapten is by itself not sufficient to elicit an antibody response alone, but requires a protein to do so.

A hapten is only antigenic when it is coupled with which of the following:

3. To make a 1 : 10 dilution, 270 µL of diluent should be added to 30 µL of sample, .

A spinal fluid that is slightly hazy is briefly examined microscopically. The technologist performing the count decides to make a 1:10 dilution using 30 µL of sample.

What volume of diluent should be used?

4. What is the best description of the purpose of the Commission on Office Laboratory Accreditation (COLA) pertaining to the clinical laboratory?

5. PCT usually rises within 3-6 hours of infection. CRP also increases rapidly following infection, but is not as specific for infection as PCT. A rise in CRP could also occur with SIRS. Lactic acid is usually used to detect and monitor impaired circulation and tissue oxygenation in critically ill patients.


Of the three laboratory tests that are listed, which has proven to be most effective for early differentiation of systemic inflammatory response syndrome (SIRS) from sepsis due to its increase following infection and higher specificity?

6. Most researchers believe that the small size and increased density of LDL molecules found in atherogenic dyslipidemia enable the molecules to more easily invade the endothelium and arterial wall of vessels.

Why are small dense LDL molecules more atherogenic?

7. Molarity x Molecular Weight x Volume = Grams

Molecular Weight (aka Formula Weight) =

2(1) + 32 + 4(16) = 98

So, 4 x 98 x 0.2L = 78.4g

What weight of H2SO4 is contained in 200 ml of a 4 molar H2SO4 solution? (Atomic weight: H= 1; S = 32; 0 = 16)

8. Shigella and Salmonella are not found as normal flora in the gastrointestinal tract.

Which of the following are not considered normal flora of the gastrointestinal tract:

9. The identification of the crystals depicted in this image are triple phosphate crystals. Triple phosphate crystals may be normal but are usually associated with alkaline urine. These colorless crystals have a characteristic "coffin lid" appearance.

What is the identification of these crystals seen in urine with an alkaline pH?

10. Though automated extraction machines have many benefits over manual methods the costs are high and generally require a high throughput of samples in order to justify the costs.

Automated extraction has many benefits over the traditional manual methods. The most important benefit is that the nucleic acid isolated is constantly consistent. There is a reduced amount of manipulation with dramatically decreases the chance of cross contamination. Also, automated extraction machines are considered moderate complexity

and can be performed by a wider variety of laboratory professionals.

All of the following are considered benefits of automated isolation and extraction equipment



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